Contact Information:

Office 1308N (tel 212-772-5383), Lab 1318N (tel 212-650-3917), dept fax 212-772-5332, email dgoss@hunter.cuny.edu, group webpage Goss Group Homepage

Education:

B.S. Nebraska Wesleyan University, Ph.D. University of Nebraska, Postdoc University of Georgia; University of Nebraska

Research Summary: 

Our lab is using biophysical methods and molecular biology to study protein-nucleic acid interactions and macromolecular assembly. We are particularly interested in mechanisms of assembly and how kinetics and equilibria influence the final composition of assembly complexes and ultimately biological function. Experiments are aimed at determining the detailed function of proteins and how they recognize nucleic acids. These processes are studied by steady-state and life-time fluorescence measurements, circular dichroism, rapid scanning stopped-flow, and site-directed mutagenesis. Detailed quantitative measurements of these biological processes lead to elucidation of molecular mechanisms and how these processes can be regulated.

Selected Publications

Ecevit, O. Khan, M.A. and Goss, D.J. (2010) "Kinetic analysis of the interaction of b/HLH/z transcription factors Myc, Max, and Mad with cognate DNA"  Biochemistry 30: 2627-35.

Khan, M. A., Yumak, H. and Goss, D.J. (2009) "Kinetic mechanism for the binding of eIF4F and tobacco etch virus internal ribosome entry site RNA: efects of eIF4B and poly(A) binding protein" J. Biol. Chem. 284: 35461-70.

Theil, E.C. and Goss, D.J. (2009) "Living with iron (and oxygen): questions and answers about iron homeostasis" Chem Rev. 109: 4568-79.

Khan, M. A., Walden, W.E., Goss, D.J. and Theil, E.C. (2009) "Direct Fe2+ sensing by iron-responsive messenger RNA:repressor complexes weakens binding" J. Biol. Chem. 284: 30122-8.

Cheng, S., Sultana, S. Goss, D.J. and Gallie, D.R. (2008) "Translation initiation factor 4B homodimerization, RNA binding, and interaction with Poly(A)-binding protein are enhanced by zinc" J. Biol. Chem. 283: 36140-53.

Baldwin, A.E., Khan, M.A., Tumer, N.E., Goss, D.J. and Friedland, D.E. (2009) "Characterization of pokeweed antiviral protein binding to mRNA cap analogs: competition with nucleotides and enhancement by translation initiation factor iso4G" Biochim Biophys Acta 1789:109-16.

Khan, M.A., Yumak, H., Gallie, D.R. and Goss, D.J. (2008) "Effects of poly(A)-binding protein on the interactions of translation initiation factor eIF4F and eIF4f-4B with internal ribosome entry site (IRES) of tabacco etch virus RNA" Biochim Biophys Acta 1779: 622-7.

Khan, M.A., Miyoshi, H.Gallie DR and Goss, D.J. (2008) "Potyvirus genome-linked protein, VPg, directly affects wheat germ invitro translation: interactions with translation initiation factors eIF4F and eIFiso4F" J. Biol. Chem. 283: 1340-9.