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NMR SPECTROSCOPY FACILITY
Director: Matthew Devany, Ph.D., Room: 1302N, Phone: (212) 772-5337, Fax: (212) 772-5332, Email: mdevany@hunter.cuny.edu
Download: Bruker 500 protocol
Background and Overview
The NMR Facility at Hunter was established in 1983 and has recently undergone major renovations. At present, the facility consists of four NMR instruments: a JEOL GX-400, a Varian Inova 500, a Bruker Avance 500 equipped with a 13C-1H cryoprobe, and a Bruker Avance III 600 MHz spectrometer equipped with a cryoprobe. These spectrometers are utilized by scientists from Hunter College, as well as from the entire CUNY community. The large variety of available probes allows detection of virtually any MR-active nuclide. Data analysis is performed either at the spectrometer workstation with vendor software or off-line with third party software packages. The Facility gets extensive use from researchers for the analysis of organic and inorganic compounds, for the identification of natural products, and for the determination of three-dimensional structures of macromolecules.
Philosophy
Rather than acting as a service facility, to which researchers submit samples or make requests, the NMR Facility is an integral part of the research experience at Hunter. Almost all experiments are performed on a hands-on basis by undergraduates, graduate students, postdoctoral fellows, and faculty. Researchers employ the spectrometers to perform a wide range of experiments, from "simple" proton and carbon spectra, to basic two-dimensional experiments, such as COSY and NOESY, to the latest higher dimensional sequences used in structural biology.
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| Bruker
Avance III 600MHz |
Bruker
Avance DRX 500MHz |
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| Varian
INOVA 500MHz |
JEOL
GX 400MHz |
Instrumentation
| 600 MHz Bruker Avance III |
The 600 MHz Bruker Avance III instrument is the newest addition to our NMR facility. This 1H cryoprobe-equipped four channel spectrometer is a state-of-the-art ultra-shielded magnet with an impressively small stray field, yielding increased lab safety and field homogeneity. The 1H signal-to-noise specification is ~5000:1 for this instrument. Currently, this instrument is reserved almost exclusively for the laboratory of Dr. Nancy Greenbaum for structural biology studies of macromolecules. |
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| Bruker Avance 500 MHz | This is our workhorse instrument, used by numerous researchers every day. Unique among our instruments is the carbon-proton cryoprobe of this spectrometer. This probe gives a sensitivity enhancement of a factor of four over conventional probes, for both carbon and proton observe experiments. The instrument boasts signal-to-noise ratios of ~2300:1 for proton and 1500:1 for 13C. All researchers have access to the ultra-high sensitivity which this probe provides. This probe provides the ability to perform 13C experiments in a short time frame, as well as 2D experiments on sample quantities which are too small to analyze on conventional probes. |
| JEOL GX 400 MHz | The GX-400 is a venerable instrument primarily used for NMR of metal nuclei with relaxation properties that favor low field: e.g. 31P and 183W. It has full variable temperature and multinuclear capabilities. We have a full range of probes, 5mm as well as 10mm broadband probes, broadband and inverse detect, as well as a 10mm low frequency probe. |
| Varian Inova 500 MHz | The Inova is our most versatile instrument, capable of NMR on both liquids and solids. It is equipped with Z-axis pulsed field gradients, a variety of probes, and high power amplifiers. It has been utilized for the characterization of solids, variable temperature experiments on a variety of nuclides such as 1H, 13C, 19F, and 99Tc, and also for the structure determination of peptides and proteins, making use of 1D, 2D, and 3D experiments. For comparison with our cryoprobe-equipped instruments, typical probes on this instrument achieve signal-to-noise ratios from 100:1 to 300:1 depending on the nucleus of interest. |
Charges
The cost for use of the Facility is $6/hour. This applies to researchers at Hunter or other CUNY schools. If time is available, researchers from other universities may use the Facility for the same price. The charge for industrial use is $100/hour and is allowable if this usage does not conflict with federal regulations concerning use of equipment purchased with federal funds. Interested parties are encouraged to contact the Facility Director for questions, training or scheduling.
