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Single Molecule Imaging of Endogenous mRNA in Live Cells and Tissues

Single Molecule Imaging of Endogenous mRNA in Live Cells and Tissues.

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When Apr 30, 2014
from 02:00 pm to 03:30 pm
Speaker Hye Yoon Park
Speaker Information Albert Einstein College of Medicine.
Where 1311 HN
Contact Name Hyungsik Lim
Contact Email
Contact Phone 212-772-4806
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ABSTRACT:
I will present how we could begin to understand single molecule biophysics of gene expression during brain activities. RNA (ribonucleic acid) dynamics - the synthesis, transport, and degradation - is involved in a variety of neuronal processes, such as axonal guidance, synaptogenesis, and long-term plasticity. The rationale for having to study single endogenous mRNA (messenger RNA) in live tissue is clear. Many aspects of RNA dynamics are a result of complex interactions of cells in the living brain, and an endogenous mRNA forms a very complicated machinery of mRNP (messenger ribonucleoprotein) complex which cannot be easily replicated by exogenous reporter genes and tissue cultures. Furthermore, the variants of mRNP complex can be resolved only by watching single molecules. I will describe a novel systems approach, combining single-particle tracking, genetic engineering, and intravital microscopy, which allows us to investigate for the first time the dynamics of gene expression at the level of RNA in living animals.

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